Tetraphenylethene-glycocluster camouflaged lectin B-targeted nano-photosensitizer for antimicrobial photodynamic therapy of Pseudomonas aeruginosa and infected wound healing

• • TFP2F achieved a 3-fold reduction in MIC80 from 7.5 μM (free TFP2) to 2.5 μM against drug-resistant P. aeruginosa under light irradiation, demonstrating enhanced targeting and phototoxicity. • • In a murine infected wound model, TFP2F plus light irradiation reduced wound area to <20% of initial by day 8, compared to >40% in controls, indicating accelerated healing. • • TFP2F eliminated >95% of P. aeruginosa within 2 days of treatment, showing rapid antibacterial efficacy critical for clinical infection control. • • TFP2 exhibited superior Type-I/II ROS generation, including hydroxyl radicals (·OH), superoxide (·O2−), and singlet oxygen (1O2), which are difficult for bacteria to develop resistance against.