One-step synthesis of PtRhMo/Rh nanozymes for mitochondrial damage-mediated photothermal/enzymatic therapy

• • Photothermal conversion efficiency reaches 55.6%, enabling effective NIR-induced hyperthermia at low laser power densities, which reduces off-target thermal damage to healthy tissues. • • The nanozyme exhibits five enzyme-like activities (CAT, OXD, POD, GPx, NOX), generating a cascade of ROS and depleting NADH, which impairs mitochondrial function and downregulates HSP70, overcoming tumor thermoresistance. • • In vivo biocompatibility at 400 ppm shows no hemolysis and no significant organ pathology or hematological fluctuations (Mon, RBC, HGB, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT, MPV, PDW), supporting safe systemic administration. • • The one-step synthesis method simplifies production and enhances heterostructure formation, increasing catalytic active sites and electron transfer, which is scalable for industrial manufacturing.