Golgi-Targeted Clay Nanoregulators with Spatiotemporal Thermal Confinement and Cascade-Amplified Antigen Delivery for Tumor Therapy

• • PM@CS reduces GOLPH3 and GOLM1 expression by 63.4% and 70.3%, respectively, demonstrating effective disruption of metastasis-associated protein secretion, which is critical for preventing tumor spread. • • Dendritic cell maturation (CD80+ and CD86+ populations) is increased by 3.3-fold, indicating a robust immunoadjuvant effect that enhances antigen presentation and primes adaptive immunity. • • Golgi-targeted thermal confinement minimizes heat transfer distance, overcoming the limitation of sublethal hyperthermia that causes marginal recurrence in over 60% of locally treated tumors. • • Upregulation of voltage-gated calcium channels and enhanced Ca2+ influx activate calcium signaling cascades, providing a mechanistic link between photothermal damage and amplified immunotherapy.