Application of Biotin-DFYIGSRGD Hydrogel in the Construction of an Islet Culture Platform

• • MIN6 cells cultured on Biotin-DFYIGSRGD hydrogel self-organized into spheroids, leading to increased insulin and glucagon synthesis compared to 2D plates, indicating enhanced β-cell functionality. • • The hydrogel preserved human islet integrity and extended in vitro culture period, addressing the bottleneck of limited islet viability in conventional culture. • • In vivo retention exceeded 120 days with gradual degradation and no significant inflammatory response, supporting long-term implantation for diabetes therapy. • • The gel's optimal hardness facilitated cell adhesion and promoted vascularization, critical for islet survival and function post-transplantation.