A Highly Fluorescent Tumor-Targeting Photosensitizer for DSLR Camera Image-Guided Two-Photon Photodynamic Therapy

• • TTAn demonstrated a 2800-fold higher cellular uptake than clinical photosensitizer Ce6 in Eca-109 cells, indicating exceptional tumor accumulation potential for enhanced PDT efficacy. • • Under white light irradiation, TTAn achieved an IC50 of 21 nM, which is 50-fold lower than Ce6, signifying potent photocytotoxicity at nanomolar concentrations. • • TTAn exhibited balanced generation of singlet oxygen and superoxide anion, enabling effective ROS-mediated cell death while maintaining biosafety in vivo. • • TTAn enabled fluorescence imaging of tumors in mice under both one- and two-photon excitation, detectable by smartphones and DSLR cameras, eliminating the need for sophisticated imaging systems.