• • The nanoprobe CPPO@TN NPs exhibits NIR chemiluminescence with a signal-to-noise ratio sufficient for in vivo imaging of drug-induced liver injury and peritonitis, achieving high sensitivity and specificity in murine models.
• • The system generates sustained singlet oxygen (1O2) production without external irradiation, enabling effective photodynamic therapy; in a 4T1 tumor-bearing mouse model, tumor growth was significantly inhibited.
• • Co-encapsulation of CPPO, AIE photosensitizer TN, and soybean oil within F-127 micelles ensures a persistent CIEEL process, providing prolonged chemiluminescence and 1O2 generation for theranostic applications.
• • The nanoprobe operates without external light excitation, eliminating tissue autofluorescence and photobleaching, thereby improving imaging fidelity and therapeutic safety in deep-tissue applications.